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| Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Salvadori,José Roberto; Nicolau,Marcio; Kaltchuk-Santos,Eliane; Alves,Laura Bianchessi; Bodanese-Zanettini,Maria Helena. |
| The objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anticarsia gemmatalis; Glycine max; Plant resistance to insects; Gene transfer. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700003 |
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| Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Pasquali,Giancarlo; Zaidi,Mohsin Abbas; Altosaar,Illimar; Bodanese-Zanettini,Maria Helena. |
| Somatic embryos of the commercial soybean (Glycine max) cultivar IAS5 were co-transformed using particle bombardment with a synthetic form of the Bacillus thuringiensis delta-endotoxin crystal protein gene cry1Ac, the beta-glucuronidase reporter gene gusA and the hygromycin resistance gene hpt. Hygromycin-resistant tissues were proliferated individually to give rise to nine sets of clones corresponding to independent transformation events. The co-bombardment resulted in a co-transformation efficiency of 44%. Many histodifferentiated embryos and 30 well-developed plants were obtained. Twenty of these plants flowered and fourteen set seeds. The integration and expression of the cry1Ac, gusA and hpt transgenes into the genomes of a sample of transformed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anticarsia gemmatalis; Cry1Ac gene; IAS5 cultivar; Insect resistance; Transgenic soybean. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300020 |
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| Homrich,Milena Schenkel; Wiebke-Strohm,Beatriz; Weber,Ricardo Luís Mayer; Bodanese-Zanettini,Maria Helena. |
| Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes. However, the stable transformation of soybean cannot yet be considered to be routine because it depends on the ability to combine efficient transformation and regeneration techniques. Two methods have been used with relative success to produce completely and stably transformed plants: particle bombardment and the Agrobacterium tumefaciens system. In addition, transformation by Agrobacterium rhizogenes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transgenic plants; Particle bombardment; Agrobacterium systems; Functional analysis; Genetic improvement. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015 |
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